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Journal : Jurnal Ilmiah Perikanan dan Kelautan

Aktivitas Enzimatis Isolat Bakteri Asam Laktat dari Saluran Pencernaan Kepiting Bakau (Scylla spp.) Sebagai Kandidat Probiotik [Activity Enzymatic of Isolate Lactic Acid Bacteria from the Digestive Tract of Mud Crab (Scylla spp.) as a Candidate Probiotics] Wahju Tjahjaningsih; Endang Dewi Masithah; Heru Pramono; Pipin Suciati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 8 No. 2 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1343.957 KB) | DOI: 10.20473/jipk.v8i2.11182

Abstract

Abstrak Probiotik dapat didefinisikan sebagai mikroba hidup yang ditambahkan dalam jumlah tertentu yang mampu bertahan hidup dalam ekosistem saluran pencernaan. Enzim yang dihasilkan oleh mikroba yang diisolasi dari saluran pencernaan ikan dapat digunakan sebagai probiotik. Enzim proteolitik ekstraseluler secara alami diproduksi oleh mikroba untuk menghidrolisis polipeptida dalam media menjadi peptida dan asam amino. Bakteri asam laktat dapat menghasil enzim seperti protease, α-amilase, fitase, kitinase, lipase. Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.) yang mempunyai aktivitas proteolitik. Penelitian ini menggunakan metode deskriptif berupa aktivitas enzimatis dan karakterisasi uji biokimia isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.). Jumlah kepiting bakau yang digunakan adalah 10 ekor. Hasil penelitian didapatkan tiga isolat bakteri asam laktat yang memiliki aktivitas proteolitik kuat, yaitu WK 28, WK 33, dan WK 53. Hasil uji biokimia isolat WK 28 termasuk ke dalam genus Pediococcus sp., isolat WK 33 termasuk ke dalam Lactobacillus sp., dan isolat WK 53 termasuk ke dalam genus Streptococcus sp. WK 28 (Pediococcus sp.) dan WK 33 (Lactobacillus sp.) mempunyai aktivitas proteolitik dan aktivitas lipolitik. Isolat WK 53 (Streptococcus sp.) mempunyai aktivitas enzimatis yaitu aktivitas proteolitik, amilolitik, dan lipolitik. Abstract Probiotics are defined as live microbes are added in a certain amount that is able to survive in the digestive tract ecosystem. Enzymes produced by microbes isolated from the digestive tract of fish can be used as probiotics. Extracellular proteolytic enzymes naturally produced by the microbes to hydrolyze a polypeptide in a media into peptides and amino acids. Lactic acid bacteria can produce enzymes such as proteases, α-amylase, phytase, chitinase, lipase. This study aims to get the lactic acid bacteria isolates from the gastrointestinal tract of mangrove crab (Scylla spp.) That have proteolytic activity. This research uses descriptive method such as enzymatic activity and biochemical characterization of isolates of lactic acid bacteria from the digestive tract of mangrove crab (Scylla spp.). Amount of mud crab used is 10 fish. The result showed three isolates of lactic acid bacteria that have a strong proteolytic activity, namely WK 28 WK 33 and WK 53. The results of biochemical tests WK 28 isolates belonging to the genus Pediococcus sp., Isolate WK 33 belonging to the Lactobacillus sp., And WK 53 isolates belonging to the genus Streptococcus sp. WK 28 (Pediococcus sp.) And WK 33 (Lactobacillus sp.) Have proteolytic activity and lipolytic activity. Isolates WK 53 (Streptococcus sp.) Have enzymatic activity is proteolytic activity, amylolytic, and lipolytic.
Perbandingan Morfologi Kerang Darah di Perairan Kenjeran dan Perairan Sedati [ Comparative Morphology of Blood Cockles in Kenjeran and Sedati] Kustiawan Tri Pursetyo; Wahju Thajaningsih; Heru pramono
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 1 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.402 KB) | DOI: 10.20473/jipk.v7i1.11222

Abstract

Abstract Blood cockles (Anadara antiquata, Anadara granosa) and Ark clams (Trisidos tortuosa) is one type of clams that is often consumed by people in Surabaya and Sidoarjo. The study was conducted in February-April 2014. The ratio of N/P on water conditions in Sedati of 3.3 / 1 it’s indicates that the sampling process is done in the rainy season which resulted in the case of stirring the sediment. On the condition of the ratio N/P is phytoplankton still able to grow optimally. While the study site in Kenjeran, Surabaya obtained ratio N/P by 5.56 / 1 this indicates the potential of phytoplankton at this location is more optimal than the region in Sedati. In morphology, clams from Kenjeran is relatively larger than the clams from Sedati.
Isolasi dan Identifikasi Vibrio sp. pada Produk Seafood Tradisional Area Timur Kota Surabaya [Isolation and Identification of Vibrio sp. from Traditional Seafood Products of Eastern Surabaya City Area] Heru Pramono; Hafizh M. Noor; Siti Sahatul Fatimah; Nur Aida Harahap; Alief Ayu Selia
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 1 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.387 KB) | DOI: 10.20473/jipk.v7i1.11223

Abstract

Abstrak Vibrio sp. adalah patogen oportunis yang terdapat pada ekosistem muara dan lingkungan laut. Vibrio sp. Dapat menyebabkan gastroenteritis dan septikemia sedangkan V. alginolyticus memiliki peran penting dalam pembusukan produk seafood. Keberadaan Vibrio sp. dalam produk olahan seafood perlu diperhatikan karena salah satu agen kausatif penyebab food-borne illness. Tujuan penelitian ini adalah untuk mengisolasi dan mengidentifikasi Vibrio spp. Pada produk olahan seafood tradisional daerah Surabaya Timur. Total 24 sampel telah dibeli dari pasar ikan tradisional di seluruh wilayah Surabaya Timur, yang diolah dengan menggunakan sepuluh teknik yang berbeda. Dua belas isolat dengan ukuran koloni yang berbeda dan warna diisolasi dan didasarkan pada identifikasi sebelumnya, terdapat sembilan Vibrio sp. Hasil uji biokimia menunjukkan bahwa terdapat delapan isolat Vibrio alginolyticus dan satu V. parahaemolyticus. Keberadaan Vibrio sp. dalam produk olahan seafood tradisional harus menjadi perhatian sebelum karena produk tersebut siap untuk makan dan dapat sumber wabah penyakit yang disebabkan oleh makanan.
Aplikasi Teknologi Asap Cair dalam Pengolahan dan Pengawetan Produk Perikanan di Pulau Mandangin [ Technology Application of Liquid Smoke in Processing and Preservation of Fishery Products in The Mandangin Island] Sapto Andriyono; Wahju Thajaningsih; Agustono Agustono; Endang Dewi Masithah; Kustiawan tri Pursetyo; Annur Ahadi Abdillah; Heru Pramono
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 1 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.185 KB) | DOI: 10.20473/jipk.v7i1.11228

Abstract

Abstract The increase in value-added of fishery products is one of which can be done by processing. In addition to maintaining the value of the protein in the fish raw material, it would be easier if the product is stored and increase the sale value of the fish itself. The ability to perform in the fish processing in Mandangin island is still very limited. Community service activities that have been carried out by a team of the Faculty of Fisheries and Marine Universitas Airlangga was introduce smoked fish product processing with liquid smoke,because this method easier and faster so that the products can be marketed. Liquid smoke was developed because the location is very minimal in the supply of raw materials such as firewood and coconut, so the use of liquid smoke is very appropriate. The resulting refined products are expected to be a source of protein Mandangin Island community itself and is able to become an alternative source of income for the community to increase the sale value of fishery products. Mentoring process being conducted by a team from the Faculty of Fisheries and Marine Universitas Airlangga so that the products can be marketed outside Mandangin Island or even to other areas in East Java.